|
|
楼主 |
发表于 2021-6-20 18:02:59
|
显示全部楼层
本帖最后由 青龙偃月刀 于 2021-6-21 08:53 编辑
纳米抗体作为一个研究工具
作为研究工具的多功能亲和试剂应该很容易产生，靶标特异性好。
有时，亲和试剂需要在不影响靶标的情况下识别所有的构象结构，也可能只识别某一个构象结构，从而能调节和影响到靶分子的功能。好的亲和试剂在不同的细胞室里发挥功能。
reagent should be functioning in different cellular compartments.
Nbs like many other affinity reagents fit perfectly
in this picture. Their high specificity for one
particular conformation of the Ag makes that the crystallisation
of the Nb-Ag complex is highly successful,
even for dynamic molecules [103]. As a result, well over
200 protein crystal structures were solved, of which the
vast majority of proteins failed to have their structure
determined at high resolution [114]. Nbs were also
instrumental to identify the interaction side of subdomains
of large proteins or to investigate in real time the
protein–protein interactions within a cell and for superresolution
microscopy [94,115,116]. The function of
native enzymes has been knocked out or modified, even
intracellularly, after direct Nb binding, by mediating the
proteolysis of the Ag [97,99], by its rerouting to other
cellular compartments or by keeping the Ag at a location
where it is not functioning [101,117,118]. Such functional
knockouts are of significant interest in cell
biology or in unveiling the life cycle of infectious pathogens.
Also tracking and the study of the dynamics of the
Ag inside cells became feasible with chromobodies or
LlamaTags, obtained after intracellular expression of a
Nb-fluorescent protein gene construct [92,119].
|
|
发表于 2021-6-12 15:21:24
